Abstract:
Data on the growth rates and specific cellulase activities of 10 isolates of the fungus Pseudogymnoascus roseus from sub- and maritime Antarctica, exposed in vitro to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius and their constant mean temperatures of 9.0 degrees Celsius, 10.7, 12.0 or 13.1 degrees Celsius, respectively. The 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia, The Backslope on Signy Island, Walton Terraces on Leonie Island and Mars Oasis on Alexander Island.
Funding:
UK Natural Environment Research Council (core funding to the British Antarctic Survey).
*** PLEASE BE ADVISED TO USE VERSION 2.0 OF THIS DATASET ***
This dataset has been superseded by Version 2.0 (https://data.bas.ac.uk/full-record.php?id=GB/NERC/BAS/PDC/01807 - see 'Related Data Set Metadata' link below). Version 2.0 has been updated to include additional data from a third experiment and to update the metadata accordingly
Keywords:
Antarctic heatwaves, beta-glucosidase , cellulase, fluctuating temperature, growth rate, psychrotrophy , soil fungi
Newsham, K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3ef187b8-3471-42ab-bc5d-d7e5e6f5d575
| Access Constraints: | Under embargo until publication of associated article. |
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| Use Constraints: | Data released under Open Government Licence V3.0: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ Use the following citation: Newsham, K. K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3EF187B8-3471-42AB-BC5D-D7E5E6F5D575 |
| Creation Date: | 2023-04-28 |
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| Dataset Progress: | Complete |
| Dataset Language: | English |
| ISO Topic Categories: |
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| Parameters: |
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| Personnel: | |
| Name | UK Polar Data Centre |
| Role(s) | Metadata Author |
| Organisation | British Antarctic Survey |
| Name | Dr Kevin K Newsham |
| Role(s) | Investigator |
| Organisation | British Antarctic Survey |
| Parent Dataset: | N/A |
| Reference: | Associated paper: Newsham, K.K. (in prep). Fluctuating temperature inhibits the growth of a widespread Antarctic fungus. Misiak, M., Goodall-Copestake, W.P., Sparks, T.H., Worland, M.R., Boddy, L., Magan, N., et al. (2021). Inhibitory effects of climate change on the growth and extracellular enzyme activities of a widespread Antarctic soil fungus. Global Change Biology, 27, 1111-1125. Doi: 10.1111/gcb.15456 |
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| Quality: | Data have not been cleaned. Data losses, indicated by NaN were caused by laboratory errors. Note that the accuracy of growth rate data measured at 12 degrees Celsius and 2-21 degrees Celsius over 0-48 hours was lower than for other growth rate data. | |
| Lineage: | A total of 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3). They were maintained on half-strength potato dextrose medium. Isolates were then inoculated onto soil extract medium in Petri dishes, which were incubated at temperatures fluctuating daily from 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius, and at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius, respectively. The constant temperatures were the means of the fluctuating temperatures (i.e., 9.0 degrees Celsius was the mean of the temperatures fluctuating from 2-15 degrees Celsius, 10.7 degrees Celsius was the mean of the temperatures fluctuating from 2-18 degrees Celsius, 12.0 degrees Celsius was the mean of the temperatures fluctuating from 2-21 degrees Celsius, and 13.1 degrees Celsius was the mean of the temperatures fluctuating from 2-24 degrees Celsius). Four replicate dishes per isolate were incubated at each temperature. Radial hyphal extension rates of colonies (referred to as growth rates) were recorded in one experiment at 15 x magnification under a dissecting microscope at 0-48 hours and were expressed as mm / day. Growth rates were also measured in another experiment, again at 15 x magnification, every 3-5 days at 7-28 days after inoculation, and were calculated by regressing colony radii against time (r2 of all fits more than or equal to 95 percent). At 28 d, extracellular beta-glucosidase (cellulase) was extracted from colonies in 10 mM phosphate buffer. Protein concentrations in the buffer were measured using a Micro BCA Protein Assay (Thermofisher). Cellulase activity was measured and expressed as nmol of p-nitrophenol released from 4-nitrophenyl-beta-D-glucopyranoside min-1 micro-grammes-1 protein (Misiak et al., 2021). | |
| Temporal Coverage: | |
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| Start Date | 2021-09-01 |
| End Date | 2023-03-31 |
| Spatial Coverage: | |
| Latitude | |
| Southernmost | -71.87833 |
| Northernmost | -54.26472 |
| Longitude | |
| Westernmost | -68.35778 |
| Easternmost | -36.49833 |
| Altitude | |
| Min Altitude | N/A |
| Max Altitude | N/A |
| Depth | |
| Min Depth | N/A |
| Max Depth | N/A |
| Location: | |
| Location | N/A |
| Detailed Location | Lewis Pass, South Georgia |
| Location | N/A |
| Detailed Location | The Backslope, Signy Island |
| Location | N/A |
| Detailed Location | Walton Terraces, Leonie Island |
| Location | N/A |
| Detailed Location | Mars Oasis, Alexander Island |
| Data Collection: | Micro BCA Protein Assay (Thermofisher) |
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| Distribution: | |
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| Distribution Media | N/A |
| Distribution Size | 14 KB |
| Distribution Format | N/A |
| Fees | N/A |
| Data Storage: | The dataset consists of one .csv file and 1 .txt file. File consists of 1,040 data points in 40 rows and 26 columns: Column A - isolate - soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3) Column B - replicate Column C - growth rate (mm / day) over 0-48 hours at 9 degrees Celsius Column D - growth rate (mm / day) over 0-48 hours at 2-15 degrees Celsius Column E - growth rate (mm / day) over 7-28 days at 9 degrees Celsius Column F - growth rate (mm / day) over 7-28 days at 2-15 degrees Celsius Column G - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 9 degrees Celsius Column H - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 2-15 degrees Celsius Column I - growth rate (mm / day) over 0-48 hours at 10.7 degrees Celsius Column J - growth rate (mm / day) over 0-48 hours at 2-18 degrees Celsius Column K - growth rate (mm / day) over 7-28 days at 10.7 degrees Celsius Column L - growth rate (mm / day) over 7-28 days at 2-18 degrees Celsius Column M - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 10.7 degrees Celsius Column N - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 2-18 degrees Celsius Column O - growth rate (mm / day) over 0-48 hours at 12 degrees Celsius Column P - growth rate (mm / day) over 0-48 hours at 2-21 degrees Celsius Column Q - growth rate (mm / day) over 7-28 days at 12 degrees Celsius Column R - growth rate (mm / day) over 7-28 days at 2-21 degrees Celsius Column S - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 12 degrees Celsius Column T - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 2-21 degrees Celsius Column U - growth rate (mm / day) over 0-48 hours at 13.1 degrees Celsius Column V - growth rate (mm / day) over 0-48 hours at 2-24 degrees Celsius Column W - growth rate (mm / day) over 7-28 days at 13.1 degrees Celsius Column X - growth rate (mm / day) over 7-28 days at 2-24 degrees Celsius Column Y - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 13.1 degrees Celsius Column Z - cellulase activity (nmol p-nitrophenol / min / microgram protein) at 28 days at 2-24 degrees Celsius |