Abstract:
Data on the growth rates and specific cellulase activities of 10 isolates of the fungus Pseudogymnoascus roseus from sub- and maritime Antarctica, exposed in vitro to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius and their corresponding mean constant temperatures of 9.0 degrees Celsius, 10.7, 12.0 or 13.1 degrees Celsius, respectively, and constant temperatures of 2 degrees Celsius, 5.5 degrees Celsius, 8.5 degrees Celsius, 15.0 degrees Celsius, 18.0 degrees Celsius, 21.0 degrees Celsius and 24.0 degrees Celsius.
Funding:
UK Natural Environment Research Council (core funding to the British Antarctic Survey).
Keywords:
Antarctic heatwaves, beta-glucosidase , cellulase, fluctuating temperature, growth rate, psychrotrophy , soil fungi
Newsham, K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures - VERSION 2.0 (Version 2.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/cf3133fb-0bd5-48e5-a89c-d464bd5b90a3
| Access Constraints: | Under embargo until publication of associated article. |
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| Use Constraints: | Data released under Open Government Licence V3.0: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ Use the following citation: Newsham, K. K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3EF187B8-3471-42AB-BC5D-D7E5E6F5D575 |
| Creation Date: | 2023-12-12 |
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| Dataset Progress: | Complete |
| Dataset Language: | English |
| ISO Topic Categories: |
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| Parameters: |
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| Personnel: | |
| Name | UK Polar Data Centre |
| Role(s) | Metadata Author |
| Organisation | British Antarctic Survey |
| Name | Dr Kevin K Newsham |
| Role(s) | Investigator, Technical Contact |
| Organisation | British Antarctic Survey |
| Parent Dataset: | N/A |
| Reference: | Associated paper: Newsham, K.K. (in prep). Fluctuating temperature inhibits the growth of a widespread Antarctic fungus. Misiak, M., Goodall-Copestake, W.P., Sparks, T.H., Worland, M.R., Boddy, L., Magan, N., et al. (2021). Inhibitory effects of climate change on the growth and extracellular enzyme activities of a widespread Antarctic soil fungus. Global Change Biology, 27, 1111-1125. Doi: 10.1111/gcb.15456 |
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| Quality: | Data have not been cleaned. Data losses, indicated by MD (missing data) were caused by laboratory errors. Note that the accuracy of growth rate data measured at 12 degrees Celsius and 2-21 degrees Celsius over 0-48 hours was lower than for other growth rate data. | |
| Lineage: | A total of 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3). They were maintained on half-strength potato dextrose medium. Three experiments were conducted. In the first, each isolate was inoculated onto soil extract medium in eight Petri dishes, which were incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony fronts were then marked on dish reverses with a fine blade at × 10-15 magnification (accuracy c. 100 micrometers) and four dishes inoculated with each isolate were transferred to cabinets fluctuating daily from 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius. The other four dishes inoculated with each isolate were transferred back to the cabinets running at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius. Note that the constant temperatures were the means of the fluctuating temperatures (e.g., 9.0 degrees Celsius was the mean of the temperatures fluctuating from 2-15 degrees Celsius). Colony fronts were marked on dish reverses 24 hours and 48 hours later, and mean growth rate (mm / day) was calculated. In the second experiment, soil extract medium in eight Petri dishes was again inoculated with each of the 10 isolates, which were again incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony radii were then measured twice at right angles to each other at × 10-15 magnification. Four dishes per isolate that had been incubated at each of these temperatures were then placed into cabinets set to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius or 2-24 degrees Celsius, respectively, and the remaining four dishes per isolate were replaced in the cabinets set to constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius. Colony radii were measured every 3-5 days over the next 21 days and mean growth rates (mm / day) were calculated by regressing colony radii against time. After 21 d, extracellular beta-glucosidase (cellulase) was extracted from colonies in 10 mM phosphate buffer. Protein concentrations in the buffer were measured using a Micro BCA Protein Assay (Thermofisher). Cellulase activity was measured and expressed as nmol of p-nitrophenol released from 4-nitrophenyl-beta-D-glucopyranoside / min / microgammes protein (Misiak et al. 2021, doi: 10.1111/gcb.15456). In the third experiment, the 10 isolates were each inoculated onto soil extract medium in four Petri dishes, which were incubated at constant temperatures of 2.0 degrees Celsius, 5.5 degrees Celsius, 8.5 degrees Celsius, 15.0 degrees Celsius, 18.0 degrees Celsius, 21.0 degrees Celsius and 24.0 degrees Celsius for one week. Colony fronts were then marked at × 10-15 magnification on dish reverses as described above. The fronts were marked again after 24 hours and 48 hours and radial extension rate (mm / day), averaged over 48 hours, was calculated. This dataset was updated to include additional data from the third experiment described above and to update the metadata accordingly. |
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| Temporal Coverage: | |
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| Start Date | 2021-09-01 |
| End Date | 2023-03-31 |
| Spatial Coverage: | |
| Latitude | |
| Southernmost | -71.87833 |
| Northernmost | -54.26472 |
| Longitude | |
| Westernmost | -68.35778 |
| Easternmost | -36.49833 |
| Altitude | |
| Min Altitude | N/A |
| Max Altitude | N/A |
| Depth | |
| Min Depth | N/A |
| Max Depth | N/A |
| Location: | |
| Location | N/A |
| Detailed Location | Lewis Pass, South Georgia |
| Location | N/A |
| Detailed Location | The Backslope, Signy Island |
| Location | N/A |
| Detailed Location | Walton Terraces, Leonie Island |
| Location | N/A |
| Detailed Location | Mars Oasis, Alexander Island |
| Data Collection: | Micro BCA Protein Assay (Thermofisher) |
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| Distribution: | |
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| Distribution Media | N/A |
| Distribution Size | 12.7 KB |
| Distribution Format | N/A |
| Fees | N/A |
| Data Storage: | The dataset consists of one CSV file, as follows. File consists of 1,320 data points in 40 rows and 33 columns: Column A - isolate Column B - replicate Column C - growth rate (mm / day) over 48 hours at 9 degrees Celsius Column D - growth rate (mm / day) over 48 hours at 2-15 degrees Celsius Column E - growth rate (mm / day) over 21 days at 9 degrees Celsius Column F - growth rate (mm / day) over 21 days at 2-15 degrees Celsius Column G - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 9 degrees Celsius Column H - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 2-15 degrees Celsius Column I - growth rate (mm / day) over 48 hours at 10.7 degrees Celsius Column J - growth rate (mm / day) over 48 hours at 2-18 degrees Celsius Column K - growth rate (mm / day) over 21 days at 10.7 degrees Celsius Column L - growth rate (mm / day) over 21 days at 2-18 degrees Celsius Column M - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 10.7 degrees Celsius Column N - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 2-18 degrees Celsius Column O - growth rate (mm / day) over 48 hours at 12 degrees Celsius Column P - growth rate (mm / day) over 48 hours at 2-21 degrees Celsius Column Q - growth rate (mm / day) over 21 days at 12 degrees Celsius Column R - growth rate (mm / day) over 21 days at 2-21 degrees Celsius Column S - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 12 degrees Celsius Column T - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 2-21 degrees Celsius Column U - growth rate (mm / day) over 48 hours at 13.1 degrees Celsius Column V -growth rate (mm / day) over 48 hours at 2-24 degrees Celsius Column W - growth rate (mm / day) over 21 days at 13.1 degrees Celsius Column X - growth rate (mm / day) over 21 days at 2-24 degrees Celsius Column Y - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 13.1 degrees Celsius Column Z - cellulase activity (nmol p-nitrophenol / min / microg protein) at 21 days at 2-24 degrees Celsius Column AA - growth rate (mm / day) over 48 hours at 2 degrees Celsius Column AB - growth rate (mm / day) over 48 hours at 5.5 degrees Celsius Column AC - growth rate (mm / day) over 48 hours at 8.5 degrees Celsius Column AD - growth rate (mm / day) over 48 hours at 15 degrees Celsius Column AE - growth rate (mm / day) over 48 hours at 18 degrees Celsius Column AF - growth rate (mm / day) over 48 hours at 21 degrees Celsius Column AG - growth rate (mm / day) over 48 hours at 24 degrees Celsius |