Abstract:
This dataset set describes the developmental stages and the molecular barcoding of Terebellidae larvae identified from diver-collected samples on 2021-04-22 in Ryder Bay near Rothera Research Station, Antarctic Peninsula, Antarctica. Photographs were collected at seven stages across the larval development. Development was captured from the trochophore stage where the polychaete larvae were bound in mucus to the free swimming planktonic metatrochophore to the nectochaete stage. Molecular barcoding was performed using the 18S rRNA gene at the British Antarctic Survey.
Funding:
This study was funded by core funding to UKRI NERC-BAS.
Keywords:
Terebellidae, larval development, metatrocophore, nectochaete, trocophore
Frontier, N., Clark, M.S., & Peck, L.S. (2024). Development and molecular barcoding of Terebellidae polychaetes from Rothera Research Station, Antarctic Peninsula, Antarctica (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/b354cde1-8cc6-4b29-a1b2-e21fa3e412e9
| Access Constraints: | No restrictions apply. |
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| Use Constraints: | Data supplied under Open Government Licence v3.0 http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/. |
| Creation Date: | 2024-07-22 |
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| Dataset Progress: | Complete |
| Dataset Language: | English |
| ISO Topic Categories: |
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| Parameters: |
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| Personnel: | |
| Name | UK Polar Data Centre |
| Role(s) | Metadata Author |
| Organisation | British Antarctic Survey |
| Name | Ms Nadia Frontier |
| Role(s) | Investigator, Technical Contact |
| Organisation | British Antarctic Survey |
| Name | Prof Melody S Clark |
| Role(s) | Investigator |
| Organisation | British Antarctic Survey |
| Name | Prof Lloyd S Peck |
| Role(s) | Investigator |
| Organisation | British Antarctic Survey |
| Parent Dataset: | N/A |
| Lineage: | Egg samples were collected on 2021-04-22 from Troval dive site, Ryder Bay, using SCUBA. Specimens were maintained in ambient water temperature during transit from the dive site to the Aquarium. Egg samples were transferred into a through-flow aquarium tank where they remained for seven months. Specimens were checked each week and photographed upon noticeable changes in their development. In the Bonner Laboratory aquarium, a subsample of the egg mass was pipetted from the mucus mass and transferred to a petri dish. A portable dissection microscope (Meiji EMZ-TR) was temporarily installed in the aquarium. Photographs were collected using a simple system of aligning an Olympus TG4 camera in the lens viewer, in the absence of a suitable aquarium microscope with a fitted camera. Samples were quickly returned to the aquarium tank. Molecular barcoding using the 18SrRNA gene was performed to identify the larvae. Briefly DNA was extracted from the larvae using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions. Amplification of a 560bp fragment of the 18S rRNA was performed using the primers NSF4 (CTGGTTGATYCTGCCAGT) and NSR581 (ATTACCGCGGCTGCTGGC) and 2x MyTaq HS Readymix (Meridian Biosciences) according to manufacturer's instructions. PCR cycling conditions were as follows: 95 degrees C for 5 mins, the 35 cycles of 95 degrees C for 20 secs; 60 degrees C for 20 secs; 72 degrees C for 30 secs and a final extension of 72 degrees C for 10 mins. The PCR product was Sanger sequenced by Source Bioscience (UK). Blast sequence similarity searching revealed that it was highly likely that these larvae were an uncharacterised Terebellidae species. In the absence of any associated adults for detailed taxonomy, more detailed identification to species level was not possible. The 18SrRNA sequence is available from NCBI under Accession number PP908343. |
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| Temporal Coverage: | |
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| Start Date | 2021-04-22 |
| End Date | 2021-04-22 |
| Spatial Coverage: | |
| Latitude | |
| Southernmost | -67.59547 |
| Northernmost | -67.59547 |
| Longitude | |
| Westernmost | -68.21003 |
| Easternmost | -68.21003 |
| Altitude | |
| Min Altitude | N/A |
| Max Altitude | N/A |
| Depth | |
| Min Depth | 27 |
| Max Depth | 27 |
| Location: | |
| Location | Antarctica |
| Detailed Location | Ryder Bay, Antarctic Peninsula |
| Data Storage: | 14 photographs in the JPG format and 1 xcsv file with contextual information for each photo. |
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